84-4409-21　C1qA ((Complement Component 1, Q Subcomponent A, q Subcomponent, Alpha Polypeptide) 200ul　362330

特徴

• Reid (1974) reported a partial aa sequence of 95 residues of the 191 residues in the oxidized A chain of human subcomponent C1q.
• This region of the A chain contains a repeating sequence of glycine-X-Y, where X is often proline and Y is often hydroxyproline, for 78 residues.
• The 5 hydroxylysine residues and 5 hydroxyproline residues in the oxidized A chain are all in these 78 residues and only in the Y position of the repeating sequence.Prolonged collagenase digestion of the oxidized A chain yielded a large, apparently C-terminal peptide containing most of the noncollagenous sequences present in the chain.
• Reid (1974) concluded that the A chain of C1q contains a collagen-like region that constitutes most of the N-terminal half of the chain.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- EDVCRAPN GKDGVAGIPG RPGRPGLKGE RGEPGAAGIR TGIRGLKGDM GESGPPGKPG NVGFPGPTGP LGNSGPQGLK GVKGNPGNIR DQPRPAFSAI RQNPPTYGNV VVFDKVLTNQ ENPYQNRTGH FICAVPGFYY FTFQVISKWD LCLSIVSSSR GQPRNSLGFC DTNSKGLFQV LAGGTVLQLQ RGDEVWIEKD PAKGRIYQGT EADSIFSGFL IFPSA
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.