BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells.
Like PD1 and CTLA4 , BTLA interacts with a B7 homolog, B7H4.
However, unlike PD-1 and CTLA-4, BTLA displays T-Cell inhibition via interaction with tumor necrosis family (TNF) receptors, not just the B7 family of cell surface receptors.
BTLA is a ligand for tumor necrosis factor (ligand) superfamily, member 14 (TNFSF14), also known as herpes virus entry mediator (HVEM).
Northern blot analysis revealed expression in lymph node and spleen, as well as in polarized Th1 but not Th2 cells.
Flow cytometric analysis demonstrated surface expression of a transmembrane protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Positive Control Included:362328A: BLTA, Positive ControlSuppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat BLTA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Lys30-Tyr183 of rat BTLA, expressed in E. coli.
