BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells.
Like PD1 and CTLA4 , BTLA interacts with a B7 homolog, B7H4.
However, unlike PD-1 and CTLA-4, BTLA displays T-Cell inhibition via interaction with tumor necrosis family (TNF) receptors, not just the B7 family of cell surface receptors.
BTLA is a ligand for tumor necrosis factor (ligand) superfamily, member 14 (TNFSF14), also known as herpes virus entry mediator (HVEM).
Northern blot analysis revealed expression in lymph node and spleen, as well as in polarized Th1 but not Th2 cells.
Flow cytometric analysis demonstrated surface expression of a transmembrane protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse BTLA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Glu30-Tyr183 of mouse BTLA, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
