The MLL is reciprocally translocated with 1 of a number of different partner genes in human acute leukemias.
The precise mechanism of oncogenic transformation is unclear since most of the partner genes encode unrelated proteins.
However, 2 partner genes, AF10 and AF17, are related through the presence of a cysteine-rich region and a leucine zipper
Overall, the protein was most closely related to the BR140 protein, and was therefore designated BRL (BR140-like gene).
Northern blot analysis revealed that a 4.6-kb BRL transcript is expressed at high levels in testis and in several cell lines.
A monoclonal antibody raised to a BRL peptide sequence confirmed its widespread expression as a 120kD protein and demonstrated localization to the nucleus of spermatocytes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human BRD1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln579-Asp1058 of human BRD1 fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
