BPI was initially identified in neutrophils, but is found in other tissues including the epithelial lining of mucus membranes.
It is an endogenous antibiotic protein with potent killing activity against some bacteria (Gram-negative bacteria).
It binds to compounds called lipopolysaccharides produced by Gram-negative bacteria.
Lipolysaccharides are potent activators of the immune system, however BPI at certain concentrations can prevent this activation
Because lipopolysaccharides are potent inflammatory agents, and the action of antibiotics can result in the release of these compounds, the binding capacity of BPI was explored as a possible means of reducing injury.
Xoma Ltd.
developed a recombinant 25kD portion of the BPI molecule called rBPI21, NEUPREX or opebecan.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse BPI.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Asn320-Pro457 of mouse BPI, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
