BMPER contains a 39aa signal peptide at the N terminus, 5 tandem cysteine-rich von Willebrand C (VWC)-like domains, 1 von Willebrand D domain, and a C-terminal trypsin inhibitor domain.
Expression of recombinant Bmper showed the presence of full-length protein and a 42kD C-terminal proteolytic fragment.
In adult mice, expression of Bmper was high in heart, lung, and skin and low in brain.
In situ hybridization of mouse tissue showed dynamic expression of Bmper during embryonic development.
At embryonic day 9.5, Bmper staining was seen in the lamina reuniens adjacent to the telencephalic vesicle and in migrating branchial arch cells.
At embryonic day 10.5, staining was seen in endothelial cells around the dorsal aorta and in adjacent migrating or mesenchymal cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse BMPER.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ser40-Ile298 from mouse BMPER fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
