Biliverdin reductases, such as BLVRA (EC 1.3.1.24), catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH.
The deduced 296aa protein has a calculated molecular mass of 33.2kD.
Removal of 2 N-terminal amino acids results in a 294aa mature protein with an N-terminal threonine.
The N-terminal region contains the NADH/NADPH-binding consensus sequence.
BLVRA shares 82.8% amino acid identity with rat Blvra, but it does not share significant homology with BLVRB.
Northern blot analysis detected BLVRA at 1.35 kb in all 8 tissues examined.
Expression was highest in brain, pancreas, and lung, and lowest in liver and placenta.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human BLVRA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met-His125 of human BLVRA, fused to two N-terminal Tags, His-Tag, GST-tag expressed in E. coli.
