BIN2 has acidic and serine/proline-rich stretches but lacks a C-terminal SH3 domain or a MYC-interacting region.
Northern blot analysis revealed expression of a major 2.6-kb transcript that was highest in spleen and peripheral blood leukocytes and also high in thymus, colon, and placenta, suggesting preferential expression in hematopoietic tissues.
Strong expression was detected in lymphoid and granulocytic cell lines but not other cell lines.
Coimmunoprecipitation and Western blot analyses showed expression of an 80kD protein that interacts with the N-terminal portion of the BAR domain of BIN1 isoforms but not with AMPH.
Immunofluorescence microscopy demonstrated cytosolic expression and lack of receptor-mediated endocytic function for BIN2.
Functional analysis showed that BIN2 lacks tumor suppressor features.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human BIN2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Asn244 of human BIN2, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
