Betaine-homocysteine methyltransferase (BHMT; EC 2.1.1.5) is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively.
This reaction is also required for the irreversible oxidation of choline.
The sequence of the predicted 406aa human BHMT is 94% identical to that of the porcine protein.
Maximal expression was observed when rats consumed diets deficient in methionine yet rich in methyl donors, such as choline, betaine, or sulfonium analogs of betaine.
BHMT2 was rapidly degraded when translated in vitro in rabbit reticulocyte lysates.
BHMT2 was stabilized by cotransfection of COS-1 cells with BHMT, and after cotransfection, BHMT2 coprecipitated with BHMT.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human BHMT.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys11-Leu314 of human BHMT, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
