The protein, also termed brain finger protein (BFP), was upregulated in P19 embryonic carcinoma cells following retinoic acid-induced differentiation to neural-like derivatives.
The authors cloned and sequenced a rat cDNA for BFP, which was then used to screen a human genomic phage library and thus obtain human genomic clones.
Sequence analysis showed that 89% of the amino acids and 83% of the DNA residues in human and rat ZNF179/BFP are identical
Matsuda et al.
(1996) used the human genomic clones to map ZNF179 to 17p11.2 by fluorescence in situ hybridization (FISH).
They also mapped the mouse and rat genes by FISH to regions 11B and 10q22, respectively.
See also ZNF173.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat BFP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Gly73-His322 of rat BFP, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
