84-4408-66　［受注停止］BECN1 (Beclin 1, ATG6, VPS30, Coiled-Coil,Myosin-Like BCL2 Interacting Protein, ATG6 Autophagy Related 6 Homolog) 200ul　362276

特徴

• Beclin-1 is also known as autophagy-related gene (Atg) 6.
• Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagasome in autophagy.
• Human beclin encodes a novel 450aa protein containing a coiled-coil region, within which it has limited homology to myosin-like proteins.
• Human and mouse beclin share 98% amino acid sequence identity.
• Caenorhabditis elegans and Saccharomyces cerevisiae beclin homologs were also identified.
• PROSITE analysis of human beclin detected several potential glycosylation, phosphorylation, and myristoylation sites, but no other functional sequence motifs.
• Northern blot analysis of mouse and adult human tissues revealed widespread beclin expression.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NAT
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.