Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection.
Beta-carotene 15,15-prime-monooxygenase (EC 1.13.11.21) is a key enzyme in beta-carotene metabolism to vitamin A
Beta Carotene 15,15-Monooxygenase 1 a protein that shares 36.7% sequence identity with the bovine retinal pigment epithelium-specific gene Rpe65.
They found that recombinant Drosophila Bcdo catalyzes centric cleavage of beta-carotene, resulting in the formation of 2 molecules of retinal.
RT-PCR detected Bcdo expression exclusively in Drosophila head.
Bcmo1 as critical molecular player for the regulation of PPARγ activity in adipocytes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human bCMO1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly6-Pro206 of human bCMO1, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
