Muscle biopsies from the vastus lateralis were obtained before and after 90 min of cycling at 65% VO2max in 12 women and 12 men.
The women performed the exercise bout during the follicular (FOL, day 7–9) and luteal (LUT, day 19–21) phases.
The men performed the exercise bout after eight days of ES (2 mg/d) and placebo (PL) supplementation, following a randomized, double-blind, cross-over design.
Menstrual phase and ES had no influence on the mRNA content of AST, BCKD and BCKDK.
Inactivating mutations in the BCKDK gene, which codes for the kinase responsible for the negative regulation of the branched-chain alpha-keto acid dehydrogenase complex (BCKD), have recently been associated with a form of autism in three families.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat BCKDK.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Arg44-Thr223 of rat BCKDK, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
