Neurocan is a developmentally regulated CSPG that shares functional domains with the cartilage CSPG aggrecan and the fibroblast CSPG versican.
Yamada et al.
(1994) described the molecular cloning of a novel bovine brain proteoglycan of the aggrecan/versican/neurocan family.
Since it is the shortest core protein in this family, they named it brevican (from the Latin word 'brevis,' meaning 'short')
A significant amount of brevican devoid of any glycosaminoglycan chains is present in brain, indicating that brevican is a 'part-time' proteoglycan.
Northern blot analysis demonstrated that a single 3.3-kb brevican transcript is present predominantly in the brain and that it is expressed in primary cerebellar astrocytes but not in neurons.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse BCAN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Asp23-Arg353 of mouse BCAN, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
