By subtractive hybridization screening of melanoma cell lines of different metastatic potential, Degen et al.
(1996) isolated a cDNA encoding a 260aa protein designated NMA.
Unlike calcyclin and thymosin-beta-10, which are expressed in highly metastatic lines, NMA and NMB are expressed in poorly metastatic melanoma cell lines.
The predicted NMA protein contains a signal sequence and a transmembrane domain.
Northern blot analysis revealed that expression of NMA was high in melanocytes and in 3 of 4 melanoma metastases with strong pigmentation, but low or absent in 7 other metastases.
Its expression is also high in kidney medulla, placenta, and spleen but low in kidney cortex, liver, prostate, and gut.
It is not expressed in lung, normal skin tissue, or muscle.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human BAMBI.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg29-Asp130 of human BAMBI, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
