B4GALNT2 catalyzes the last step in the biosynthesis of the human Sd(a) antigen through the addition of an N-acetylgalactosamine residue via a beta-1,4 linkage to a subterminal galactose residue substituted with an alpha-2,3-linked sialic acid.
B4GALNT2 also catalyzes the last step in the biosynthesis of the Cad antigen.
The longer transcript encodes a deduced 566aa protein with a calculated molecular mass of 63.3kD.
It contains an N-terminal cytosolic domain, followed by a transmembrane domain and a long catalytic domain.
The shorter transcript encodes a deduced 506aa protein with calculated molecular mass of 57.0kD.
It has a different N-terminal cytosolic domain than the full-length form.
Northern blot analysis detected B4GALNT2 transcripts of 8.8, 6.1, 4.7, 3.8, and 1.65 kb.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-3ug/mlImmunohistochemistry (Paraffin): 5-30ug/mlImmunocytochemistry: 5-30ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin 300, 50% glycerol.
Specificity：Recognizes human b4GALNT2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr324-Ala566 of human b4GALNT2, expressed in E. coli.
