Serine/threonine-protein kinase 13 is a member of the Aurora subfamily of serine/threonine protein kinases.
The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis.
This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction.
Alternative splicing results in multiple transcript variants.mouse Aie1 (Aurora/Ipl1/EG2-1) and human STK13, which they designated AIE2.
Both Aie1 and AIE2 contain a central serine/threonine kinase domain.
The authors reported that AIE2 contains 309aa.
Northern blot analysis detected expression of a 1.35-kb AIE2 mRNA only in testis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat AURKC.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Gly92-Pro339 of rat AURKC, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
