ATP6AP2 is associated with adenosine triphosphatases (ATPases).
Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis.
There are three classes of ATPases- F, P, and V.
The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector.
This protein has been found associated with the transmembrane sector of the V-type ATPases.Expressed in brain, heart, placenta, liver, kidney and pancreas.
Barely detectable in lung and skeletal muscles.
In the kidney cortex it is restricted to the mesangium of glomeruli.
In the coronary and kidney artery it is expressed in the subendothelium, associated to smooth muscles where it colocalizes with REN.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat ATP6AP2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Asn18-Glu302 of rat ATP6AP2, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
