ATP1a1 belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+-ATPases.
Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane.
These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle.
This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta).
The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes.
This gene encodes an alpha 1 subunit.
Alternatively spliced transcript variants encoding different isoforms have been identified.
Applications:Suitable for use in Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunocytochemistry: 5-20ug/mlImmunohistochemistry (Paraffin): 5-20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes human ATPa1. Species Crossreactivity: mouse, rat and porcine
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu Mo Po Rt
Immunogen：Recombinant protein corresponding to His493-Ala660 of human ATP1a1, expressed in E. coli.
