ATF6 is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules.
Accumulation of misfolded proteins in the Endoplasmic Reticulum results in the proteolytic cleavage of ATF6.
The cytosolic portion of ATF6 will move to the nucleus and act as a transcription factor to cause the transcription of ER chaperones.Sequence analysis predicted that the 670aa protein contains an N-terminal serine-rich domain and a central DNA-binding domain followed by a leucine zipper sequence.
Northern blot analysis revealed expression of a 2.5-kb transcript in HeLa cells and 4.5- and 8.0-kb transcripts in all mouse tissues tested, with the exception of spleen.
Immunoblot analysis detected expression of a 90kD protein in HeLa and COS cell lysates.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse ATF6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Leu377 of mouse ATF6, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
