MMSP is a rare and aggressive tumor that mainly develops in tendons and aponeuroses of patients between 15 and 35 years of age, and which may also be derived from neuroectoderm (Chung and Enzinger, 1983; Epstein et al., 1984).
Zucman et al.
(1993) demonstrated that the hybrid transcripts in the t(12;22) cases encoded a chimeric protein consisting of the N-terminal domain of EWS (133450) linked to the bZIP domain of ATF1
Zucman et al.
(1993) showed by fluorescence in situ hybridization that the ATF1 gene maps to 12q13.
Bicolor FISH on interphase nuclei from an MMSP cell line showed that the EWS locus was split and that the proximal part of EWS was juxtaposed to the ATF1 locus.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ATF1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp3-Gln213 of human ATF1, expressed in E. coli.
