Asporin, ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix.
The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin.Higher levels in osteoarthritic articular cartilage, aorta, uterus.
Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands.
Low expression in trachea, bone marrow, and lung.
Variation in the poly-Asp region of ASPN is associated with osteoarthritis susceptibility type 3 (OS3) ; also known as osteoarthritis of knee/hip.
Osteoarthritis is a common disease that produces joint pain and stiffness together with radiologic evidence of progressive degeneration of joint cartilage.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse ASPN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Lys16-Lys373 of mouse ASPN, fused to two N-terminal Tags, His-Tag, GST-tag expressed in E. coli.
