84-4407-87　ASNS (Asparagine Synthetase, Aspartate-Ammonia Ligase, Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase) 200ul　362198

特徴

• Asparagine synthetase is an enzyme that generates asparagine from aspartate.
• This amidation reaction is similar to that promoted by glutamine synthetase.human ASNS based on its ability to complement ts11, a temperature-sensitive (ts) mutant of the BHK hamster cell line that at the nonpermissive temperature is blocked in progression through the G1 phase of the cell growth cycle.
• The gene was transcribed into an mRNA of 2 kb that was expressed in all human, hamster, and mouse cell lines tested.
• It encodes a protein of about 550aa.
• The 5-prime upstream region of this gene, like other housekeeping genes, lacks conventional TATA and CAAT boxes.
• Both the human and the hamster genes have a high 5-prime G+C content which may play a role in expression through DNA methylation.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.