The ASGR2 gene encodes a glycoprotein that forms the asialoglycoprotein receptor, also known as the Ashwell receptor, which is specific for desialylated (galactosyl-terminal) glycoproteins and is expressed exclusively in hepatic parenchymal cells (Spiess and Lodish, 1985).From the same cDNA library that was made from the human hepatoma cell line HepG2 and was used to isolate ASGR1, Spiess and Lodish (1985) isolated and sequenced a clone encoding a second asialoglycoprotein receptor, which they referred to as H2 and which had protein sequence homology of 58% to H1
The rat similarly has 2 ASG receptors, R1 and R2.
Spiess and Lodish (1985) found that H1 is more homologous to R1 than to H2, and H2 is more homologous to R2 than to H1.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ASGR2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ala311 of human ASGR2, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
