84-4407-82　［受注停止］ASCL1 (Achaete Scute Complex Like Protein 1, ASH1, HASH1, MASH1, BHLHA46, Achaete Scute Homolog 1, Class A basic helix-loop-helix protein 46) 200ul　362193

特徴

• Mash1 is expressed during development of rat retina and interacts specifically with an E-box identified in the promoter for the opsin gene during rod photoreceptor differentiation.
• It encodes a predicted protein of 238aa that shares 95% identity with the mammalian achaete-scute homolog-1 (Mash1), a rodent basic helix-loop-helix factor
• The proximal coding region of the cDNA contains a striking 14-copy repeat of the triplet CAG that exhibits polymorphism in human genomic DNA; thus, ASCL1 is a candidate locus.
• Northern blot analysis revealed ASCL1 transcripts in RNA from a human MTC cell line, 2 fresh MTC tumors, fetal brain, and 3 lines of human SCLC.
• In contrast, cultured lines of non-SCLC lung cancers and a panel of normal adult human tissues showed no detectable ASCL1 transcripts.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.