ARSF is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily.
Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events.
Sulfatases are essential for the correct composition of bone and cartilage matrix.
The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin.
The ARSF cDNA encodes a 591aa polypeptide.
The gene is encoded by 2 alternative first exons (1A and 1B) followed by 9 additional exons.
Expression of the cDNA showed that the ARSF gene product is an active arylsulfatase whose activity, unlike that of the ARSE gene product, is not inhibited by warfarin.
Northern blot analysis and RT-PCR failed to detect ARSF expression.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ARSF.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to His23-Asp300 of human ARSF, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
