Arylsulfatase A hydrolyzes cerebroside sulfate to cerebroside and sulfate.
Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death.
Multiple alternatively spliced transcript variants, one of which encodes a distinct protein, have been described for this geneThe predicted amino acid sequence comprised 507 residues, including a putative signal peptide of 18 residues.
The sequence contains 3 potential N-glycosylation sites.
The cDNA hybridized to 2.0- and 3.9-kb species in RNA from human fibroblasts and human liver.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity： Recognizes human ARSA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro21-Ser295 of human ARSA, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
