ARPC2 encodes one of seven subunits of the human Arp2/3 protein complex.
The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution.
The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined.
Two alternatively spliced variants have been characterized to date.
Additional alternatively spliced variants have been described but their full length nature has not been determined.
The ARC34 cDNA encodes a deduced 300aa protein that is 34% identical to its homolog in S.
cerevisiae.
ARC34 localizes to the lamellipodia of stationary and motile fibroblasts, as well as to the actin tails assembled by Listeria monocytogenes.
ARC34 was not detected in cellular bundles of actin filaments.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ARPC2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Arg300 of human ARPC2, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
