Aquaporin-4 (AQP4), is a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells.
The encoded protein is the predominant aquaporin found in brain.
Aquaporin-4 is located in many tissues, including the basolateral membrane of the renal cortical and medullary principal cell, as well as in the brain.
Aquaporin-4 is the first specific molecule to be defined as a target for the autoimmune response in any form of multiple sclerosis.
Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells.
AQP4 is also expressed in astrocytes and is upregulated by direct insult to the central nervous system.
Aquaporin-4 has been proposed as the primary autoimmune target in Devic's disease.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat AQP4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Asp179-Asp314 of rat AQP4, fused to two N-terminal Tags, His-Tag , GST-tag expressed in E. coli.
