Apolipoprotein M is an apolipoprotein and member of the lipocalin protein family.
It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins.
The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport.
Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized.
The deduced 188aa protein has a calculated molecular mass of about 21kD.
It contains a potential N-glycosylation site, 2 possible disulfide bridges, and a hydrophobic alpha-helical signal peptide that is retained in the mature protein.
Sequence analysis revealed 79% identity between the human and mouse proteins.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes canine APOM.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ca
Immunogen：Recombinant protein corresponding to Cys23-Asn188 of canine APOM, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
