Apolipoprotein H (ApoH), also known as Beta 2-glycoprotein I, is a plasma glycoprotein either circulating as a free protein or associated to lipoproteins.
This protein was found in human serum and described for the first time in 1961 by Schultze et al.
ApoH is a 54kD single-chain glycoprotein consisting of five carbohydrate chains and 326aa residues that can be divided into five short consensus repeat domains
ApoH has been chiefly focused on as the cofactor or autoantigen in antiphospholipid syndrome (APS), which is related to venous and arterial thrombosis, fetal loss, and thrombocytopenia.
Reports show that ApoH may have an important function in blood coagulation and clearance of apoptotic bodies from the circulation.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes canine APOH.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ca
Immunogen：Recombinant protein corresponding to Arg21-Cys345 of canine APOH, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
