84-4407-49　APOE (AApolipoprotein E, Apo-E, AD2, Apoprotein, Alzheimer Disease 2, E4-Associated,Late Onset) 200ul　362160

特徴

• Apolipoprotein F is predominantly associated with low density lipoprotein and functions as an inhibitory regulator of cholesteryl ester transfer protein (CETP).
• Apolipoprotein F, a minor apolipoprotein in human plasma, was isolated and partially characterized by Olofsson et al.
• (1978).
• By PCR of a human hepatoma cell line cDNA library using primers based on the N-terminal sequence of purified APOF, Day et al.
• (1994) cloned full-length APOF.
• The deduced 308aa proprotein has a 22aa N-terminal signal peptide, and its C-terminal half contains the mature APOF peptide, which is released by proteolytic processing.
• The mature 162aa hydrophobic APOF peptide has a calculated molecular mass of 17.4kD and contains sites for N- and O-glycosylation.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QALIHHLQEL QKSGHTDREV SVDALSSALQ LLAWEQPGPK RAKRSISNTD CDNDQEQSVH NVVDLLPAVG TYYNLGTALY YAIKNCSDKA KERGRDGAID LGYDLLMAMV GASGGPAGAV ITAALKPAMK AGVQRLIQYY YDEKEVTTPQ PEVTTHQPET GKDATTDIGV VEEIAMSNFV SEVESTTSNW EWPLLKNYGV LAYK
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.