Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells.
ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents.
Apolipoprotein E has many functions in the body.
When it is synthesized by the liver as part of VLDL it functions in the transport of triglycerides to the liver tissue.
It is also incorporated into HDL (as HDL-E) and functions in cholesterol distribution among cells.
It is also incorporated into intestinally synthesized cholymicrons and transports dietary triglycerides and cholesterol.
It is involved in lipid metabolism by mediating the receptor binding of apo-E lipoproteins to the LDL receptor.
Receptor binding begins the cellular uptake of lipoproteins to be used in intracellular cholesterol metabolism.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes canine APOE.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ca
Immunogen：Recombinant protein corresponding to Asp1-Val172, of canine APOE, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
