Apolipoprotein C2 is secreted in plasma where it is a component of very low density lipoprotein.
This protein activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells.
Mutations in this gene cause hyperlipoproteinemia type IB, characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis.
Apolipoprotein C-II (apoC-II) is a necessary cofactor for the activation of lipoprotein lipase (LPL), the enzyme that hydrolyzes triglycerides in plasma and transfers the fatty acids to tissues.
The APOC2 cDNA sequence encodes a deduced 79aa protein.
Using synthetic oligonucleotides as probes, Sakaguchi et al.
(1984) isolated APOC2 from a human cDNA library.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：equine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes equine APOC2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Eq
Immunogen：Recombinant protein corresponding to Thr23-Ser101 of equine APOC2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
