APOA1BP contains an N-terminal signal peptide for translocation into the endoplasmic reticulum, a putative signal sequence cleavage site, and several putative phosphorylation and O-glycosylation sites.
The processed mature protein has a calculated molecular mass of 29.1 kD.
Human APOA1BP shares 88.2 to 89.5% amino acid identity with mouse, bovine, and porcine Apoa1bp.
RNA dot blot analysis revealed abundant APOA1BP expression in all adult and fetal tissues investigated.
Highest expression was in kidney, apex of heart, liver, thyroid gland, adrenal gland, and testis.
APOA1BP was also expressed in all cell lines examined.
APOA1BP was also detected in cerebrospinal fluid (CSF) and urine of healthy volunteers, but not in normal serum samples.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human APOA1BP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly118-Glu282 of human APOA1BP, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
