Apelin also known as APLN is a peptide which is encoded by the APLN gene.
Apelin is the endogenous ligand for the G-protein-coupled APJ receptor that is expressed at the surface of some cell types.
It is widely expressed in various organs such as the heart, lung, kidney, liver, adipose tissue,gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma
The gene encodes a 77aa polypeptide that includes a secretory signal sequence.
Synthetic 36aa apelin (apelin-36), corresponding to the most C-terminal 36 residues of preproapelin, caused the acidification of cells expressing transfected APJ, but not other receptors.
Apelin is an endogenous ligand for the APJ receptor.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse APLN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Val23-Phe77 of mouse APLN, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
