AAC11 was cloned by database analysis and screening gingiva and leukemia cDNA libraries.
The deduced 504aa human protein shares a high degree of identity with the long mouse protein.
AAC11 contains several protein phosphorylation sites, 2 N-glycosylation sites, and a leucine-rich sequence with weak similarity to a leucine zipper motif.
Northern blot analysis detected a major Aac11 transcript in all adult and embryonic mouse tissues examined and several other transcripts expressed in a tissue-specific manner.
AAC11 expression was also detected in several human tumor cell lines.
Western blot analysis of mouse fibroblasts detected a major 55kD protein and a minor 25kD protein, corresponding to the long and short Aac11 isoforms, respectively.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human API5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro2-Phe360 of human API5, fused to two N-terminal Tags, His-Tag, T7-tag expressed in E. coli.
