Amyloid beta A4 precursor protein-binding family B member 3 is a member of the APBB protein family.
It is found in the cytoplasm and binds to the intracellular domain of the Alzheimer's disease beta-amyloid precursor protein (APP) as well as to other APP-like proteins.
It is thought that the protein encoded by this gene may modulate the internalization of APP.
Multiple transcript variants encoding several different isoforms have been found for this gene
Like Fe65 and Fe65L1, the rat Fe65L2 protein contains 2 phosphotyrosine-binding (PTB) domains and a WW domain.
Northern blot analysis detected predominant expression of a 2-kb Fe65L2 mRNA in rat brain and testis.Fe65L2 encodes a deduced 486aa protein that shares 86% sequence identity with the rat protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat APBB3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Asn14-Ala302 of rat APBB3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
