The deduced 666aa protein contains an N-terminal proline-rich domain, a RAS-associated domain, a PH domain, and a long C-terminal proline-rich domain.
Northern blot analysis detected a 2.6kb transcript in most tissues, with highest expression in thymus, spleen, liver, and peripheral leukocytes.
RNA dot blot analysis detected RARP1 preferentially expressed in thymus, spleen, lymph node, bone marrow, and peripheral leukocytes.
Western blot analysis showed that endogenous promyeloleukemic cell RARP1 has an apparent molecular mass of 110kD.
Immunostaining of transfected Chinese hamster ovary cells found RARP1 localized to the cytoplasm.
Endogenous mouse Rarp1 was found in the cytoplasm of megakaryocytes or their progenitor cells in bone marrow and spleen.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human APBB1IP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser188-Lys421 of human APBB1IP fused to His-Tag and GST tag at N-terminal expressed in E. coli.
