Annexin A11 is a 56-kD antigen recognized by sera from patients with various autoimmune diseases.
Exon 1 is untranslated, and intron 1 spans about 20 kb.
The human ANXA11 gene has the same general structure as the mouse gene; however, EST database analysis indicated that human ANXA11 transcripts also use variably spliced exons 1b and 1c, which can lengthen the 5-prime UTR.
The intronic sequences of the mouse and human ANXA11 genes contain several types of repetitive elements, including retroviral long terminal repeats.
The mouse promoter region contains a remnant LINE-1 insertion not found in the human gene.
The 5-prime flanking region of the ANXA11 gene contains a CpG island and MYOD, SP1, and glucocorticoid response elements, but no proximal TATA or CAAT boxes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ANXA11.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Asp505 of human ANXA11, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
