Atrial natriuretic peptide (ANP),is a polypeptide hormone involved in the homeostatic control of body water, sodium, and adiposity.
It is released by atrial myocytes, muscle cells in the atria of the heart, in response to high blood pressure.
ANP acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure.
ANP is a 28aa peptide with a 17aa ring in the middle of the molecule.
The ring is formed by a disulfide bond between two cysteine residues at positions 7 and 23.
ANP is closely related to BNP and CNP which all share the same amino acid ring.
ANP was discovered in 1981 by a team in Ottawa led by Adolfo J.
de Bold after they made the seminal observation that injection of atrial (but not ventricular) tissue extracts into rats caused copious natriuresis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat ANP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Asn25-Arg122 of rat ANP, fused to N-terminal His-Tag expressed in E. coli.
