By searching databases for sequences similar to TMEM16A, Katoh and Katoh (2004) identified TMEM16F.
The deduced 892aa protein contains 8 transmembrane domains.
The N and C termini are cytoplasmic, and the extracellular regions contain 6 putative N-glycosylation sites.
TMEM16F and TMEM16E share 50.3% amino acid identity.
By EST database analysis, Katoh and Katoh (2004) determined that TMEM16F is expressed in embryonic stem cells, fetal liver, retina, chronic myelogenous leukemia, and intestinal cancer.
Katoh and Katoh (2004) determined that the TMEM16F gene contains 20 exons and spans more than 119.7 kb.
This locus is paralogous to the NELL1-TMEM16E locus on chromosome 11p15.1-p14.3.
Katoh and Katoh (2004) also mapped the mouse Tmem16f gene to chromosome 15E3.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Positive Control (Supplied):362122A: Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 0.02% BPB, 50mM DTT, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes human ANO6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala744-Asn824 of human ANO6, expressed in E. coli.
