84-4407-10　ANO1 (Anoctamin 1, TMEM16A, ORAOV2, DOG1, TAOS2, Oral Cancer Overexpressed 2, Tumor-amplified and overexpressed sequence 2, Discovered on gastrointestinal stromal tumors protein 1) 200ul　362121

特徴

• TMEM16A, which encodes a deduced 986aa protein.
• A shorter TMEM16A isoform results from alternative splicing.
• The human and mouse TMEM16A proteins share 89.9% sequence identity; both have 8 potential transmembrane regions with the N- and C-terminal tails facing the cytoplasm, 5 potential N-linked glycosylation sites, and a tyrosine phosphorylation site.
• TMEM16A shares 58.4%, 38.3%, and 38.6% amino acid sequence identity with C12ORF3 (TMEM16B), C11ORF25 (TMEM16C), and FLJ34272 (TMEM16D), respectively.In silico analysis indicated that TMEM16A mRNA is expressed in head and neck tumors, parathyroid tumors, and ovarian tumors, and in breast, pancreatic, gastric, and uterine cancers.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- S ADRECQRGPE TIAHEAQDAG TPNSGDATGV VDGEREATMR VPEKYSTLPA EDRSVHIVNI CAIEDLGYLP SEGTLLNSLS VDPDAECKYG LYFRDGKRKV DYILVYHHKR ASGSRTLARR GLQNDMVLGT RSVRQDQPLP GKGSPVDAGS PEVPMDYHED DKRFRREEYE GNLLEAGLEL ENDEDTKIHG VGFVKIHAPW HVLCREAEFL KLKMPTKKV
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.