Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy.
The deduced 319aa protein has a calculated molecular mass of 36kD.
ANKRD1 contains a nuclear localization signal, 4 tandem ankyrin-like repeats, multiple phosphorylation sites, and a PEST-rich region, which is typically found in rapidly degraded proteins.
The 3-prime UTR of ANKRD1 has numerous AT-rich mRNA decay elements.
Northern blot analysis of multiple human cell lines detected ANKRD1 expression in microvascular and large vessel endothelial cell lines.
Immunofluorescence of transfected COS cells and immunoprecipitation of nuclear and cytoplasmic extracts of COS cells showed that ANKRD1 was abundantly expressed in nucleus.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat ANKRD1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Arg5-Asn279 of rat ANKRD1, fused to two N-terminal Tags, His-Tag GST-tag expressed in E. coli.
