The deduced 470aa protein has an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both of which are conserved in angiopoietins.
Agf encodes a 457aa protein that shares 74% identity with human AGF.
Northern blot analysis detected 4.0- and 1.8-kb transcripts expressed abundantly in liver and weakly in heart, brain, lung, kidney, and testis.
RT-PCR showed broad expression in hematopoietic cells, with abundant expression in CD41-positive megakaryocytes/platelets, hematopoietic stem cells, and mast cells.
Western blot analysis of mouse tissues detected Agf at an apparent molecular mass of 50kD predominantly in liver.
Immunohistochemical analysis showed Agf expressed in hepatocytes.
In situ hybridization and immunohistochemical staining suggested that mast cells secrete Agf.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ANGPTL6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Trp257-Leu470 of human ANGPTL6, fused to two N-terminal Tags, His-Tag , T7-tag expressed in E. coli.
