The angiopoietins are a family of growth factors specific for vascular endothelium.
During large-scale DNA sequencing of a human fetal brain cDNA library, Zeng et al.
(2003) cloned a novel human angiopoietin-like cDNA and termed it angiopoietin-like-5 (ANGPTL5).
Like other members of the angiopoietin family, the deduced 388aa ANGPTL5 protein has an N-terminal cleavable signal peptide, a predicted coiled-coil domain, and a fibrinogen-like domain.
RT-PCR analysis detected expression of ANGPTL5 in all tissues tested, but mainly in adult human heart
Zeng et al.
(2003) determined that the ANGPTL5 gene contains 8 exons and spans approximately 17.3 kb.
By genomic sequence analysis, Zeng et al.
(2003) mapped the ANGPTL5 gene to chromosome 11q22.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ANGPTL5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg141-Tyr383 of human ANGPTL5, expressed in E. coli.
