ALDH9A1 has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes.
The enzyme catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid (GABA).
This isozyme is a tetramer of identical 54-kD subunits.
By sequence analysis of the ALDH9 locus in several unrelated individuals, Lin et al.
(1996) determined that 2 of the differences were due to polymorphism.
They attributed the third difference to a misprint in Kurys et al.
(1993).
Lin et al.
(1996) reported that the ALDH9 gene contains 10 exons and spans approximately 45 kb.
Northern blot analysis revealed that ALDH9 is expressed as a 2.4-kb mRNA in a variety of tissues, with the highest levels of expression in liver, skeletal muscle, and kidney.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse ALDH9A1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Leu322-Phe494 of mouse ALDH9A1, fused to two N-terminal Tags, His-Tag , T7-tag expressed in E. coli.
