Agrin is a large proteoglycan whose best characterised role is in the development of the neuromuscular junction during embryogenesis.
Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis.
This protein has nine domains homologous to protease inhibitors.
It may also have functions in other tissues and during other stages of development
It is major proteoglycan component in glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.
This protein binds to several receptors on the surface of skeletal muscle.
The receptor which seems to be required for formation of the neuromuscular junction (NMJ) is called the MuSK receptor (Muscle specific kinase).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse AGRN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Arg1130-Cys1401 of mouse AGRN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
