Anion Exchanger 1 is a phylogenetically preserved transport protein responsible for mediating the electroneutral exchange of chloride (Cl-) for bicarbonate (HCO3-) across a plasma membrane.It is ubiquitous throughout the vertebrates.
In humans it is present in two specific sites: the erythrocyte (red blood cell) cell membrane and the basolateral surface of the alpha-intercalated cell (the acid secreting cell type) in the collecting duct of the kidney.
The erythrocyte and kidney forms are different isoforms of the same protein.
AE1 was discovered following SDS-PAGE gel electrophoresis of erythrocyte cell membrane.
The large 'third' band on the electrophoresis gel represented AE1, which was thus initially termed 'Band 3'.
The chloride-bicarbonate exchanger in the red cell membrane is not a pump, which would use metabolic energy.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (FFPE): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin 300, 50% glycerol
Specificity：Recognizes rat AE1
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to rat AE1, expressed in E. col.
