Adiponectin, alternatively named Adipocyte Complement-Related Protein of 30kD, shares structural similarity with complement factor C1q and is a member of the family of defense collagens.
It is secreted exclusively by differentiated adipocytes and circulates at high concentrations.
Adiponectin has a modular structure comprising an N-terminal collagenous domain with multiple collagen triple helix repeats, followed by a C-terminal C1q-like globular domain.
The globular domain has similar folding topology with tumor necrosis factor-alpha and assembles into homotrimers.
Higher order oligomeric adiponectins are also formed via interactions between the collagenous stalk.
A truncated form of Adiponectin containing only the globular domain can be generated by proteolytic cleavage.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.